Mar 20, 2023

Public workspaceYT medium

DOI
dx.doi.org/10.17504/protocols.io.kxygx9qx4g8j/v1
  • 1University of Oslo
Andreas Sagen
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DOI: dx.doi.org/10.17504/protocols.io.kxygx9qx4g8j/v1
Protocol Citation: Andreas Sagen 2023. YT medium. protocols.io https://dx.doi.org/10.17504/protocols.io.kxygx9qx4g8j/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 06, 2023
Last Modified: March 20, 2023
Protocol Integer ID: 78208
Keywords: YT medium, YT, e. coli, Escherichia coli, medium, microbial growth
Abstract
2xYT is a nutritionally rich liquid microbial growth medium used for propagation of recombinant strains of Escherichia coli and M13 bacteriophage or other filamentous single-stranded DNA bacteriophages.
Guidelines
Prepare enough for the necessary number of experiments to perform in order to prevent batch-to-batch variation between experiments. Input chemicals should be of a certain quality in order to prevent contaminants that can impact microbial growth and selection.
Materials
Magnetic stirrer, autoclave, scale, tubes, flask
Protocol materials
ReagentYeast ExtractMerck MilliporeSigma (Sigma-Aldrich)Catalog #Y0875
Step 2
ReagentSodium chlorideMerck MilliporeSigma (Sigma-Aldrich)Catalog #S9625
Step 2
ReagentTryptoneMerck Millipore (EMD Millipore)Catalog #T9410
Step 2
Safety warnings
Be sure to wear appropriate PPE when working with antibiotics and chemicals. Take care when working with hot flasks and tubes.
500 mL 2x YT medium
500 mL 2x YT medium
15m
15m
Fill the bottle with Amount400 mL deionized water
5m
Measure and add:
Amount8 g Tryptone
Amount5 g Yeast extract
Amount2.5 g Sodium chloride

Powdered compounds:
ReagentTryptoneMerck Millipore (EMD Millipore)Catalog #T9410
ReagentYeast ExtractMerck MilliporeSigma (Sigma-Aldrich)Catalog #Y0875
ReagentSodium chlorideMerck MilliporeSigma (Sigma-Aldrich)Catalog #S9625
15m
Adjust pH to Ph7.0 by adding drops of concentrated NaOH
5m
Add deionized water to a total of Amount500 mL
5m
Autoclave liquid at Temperature121 °C for Duration00:15:00

Note
Cool to Temperature50 °C and supplement with antibiotics as appropriate


15m