Mar 01, 2022
Y Maze Forced Alternation
- 1University of Ottawa
Protocol Citation: Haley Geertsma 2022. Y Maze Forced Alternation. protocols.io https://dx.doi.org/10.17504/protocols.io.b5smq6c6
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: March 01, 2022
Last Modified: March 01, 2022
Protocol Integer ID: 58925
Abstract
This protocol is used to test mice in the Y Maze Forced Alternation behavioural test.
Materials
Y Maze
Habituate mice in a separate room for 60 minutes prior to testing.
1h
Confirm testing room lighting is set to 60lux and EthoVision is set up properly.
5m
Place mice in Arm 1 (with either Arm 2 or 3 blocked, alternate between mice) and record their movement for 5 minutes.
5m
After testing, place the mouse back into the home cage and clean the Y maze. Leave the mouse in its home cage for 30 minutes.
30m
Remove the blockage so all 3 arms are open and place the mouse back into Arm 1. Record their movement for 5 minutes.
5m
Once testing is complete, return mouse to its home cage and clean the Y maze.
2m