Nov 28, 2024

Public workspaceXenium: OCT

DOI
dx.doi.org/10.17504/protocols.io.4r3l29o74v1y/v1
  • 1Stanford University
  • Human BioMolecular Atlas Program (HuBMAP) Method Development Community
    Tech. support email: Jeff.spraggins@vanderbilt.edu
Joanna Bi
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DOI: dx.doi.org/10.17504/protocols.io.4r3l29o74v1y/v1
Protocol Citation: Joanna Bi 2024. Xenium: OCT. protocols.io https://dx.doi.org/10.17504/protocols.io.4r3l29o74v1y/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 28, 2024
Last Modified: November 28, 2024
Protocol Integer ID: 113019
Abstract
Xenium protocols for OCT embedded tissue.
Tissue Prep for Fresh Frozen
Tissue Prep for Fresh Frozen
Follow the attached protocols in order, starting after the tissue freezing and embedding section.
Note: The protocol is described as for fresh frozen, but involves OCT embedding.
Download Xenium In Situ for Fresh Frozen Tissues - Tissue Preparation Guide.pdfXenium In Situ for Fresh Frozen Tissues - Tissue Preparation Guide.pdf5.6MB

Download Xenium In Situ for Fresh Frozen Tissues – Fixation & Permeabilization.pdfXenium In Situ for Fresh Frozen Tissues – Fixation & Permeabilization.pdf9.1MB

Assay and Instrument
Assay and Instrument
Follow the attached protocols in order. These steps are the same for FFPE samples
Download Xenium In Situ Gene Expression - Probe Hybridization, Ligation & Amplification.pdfXenium In Situ Gene Expression - Probe Hybridization, Ligation & Amplification.pdf8MB

Download Xenium Analyzer User Guide.pdfXenium Analyzer User Guide.pdf12.2MB