Nov 27, 2024

Public workspaceXenium: FFPE

DOI
dx.doi.org/10.17504/protocols.io.kqdg3qp9pv25/v1
  • 1Stanford University
  • Human BioMolecular Atlas Program (HuBMAP) Method Development Community
    Tech. support email: Jeff.spraggins@vanderbilt.edu
Joanna Bi
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DOI: dx.doi.org/10.17504/protocols.io.kqdg3qp9pv25/v1
Protocol Citation: Joanna Bi 2024. Xenium: FFPE. protocols.io https://dx.doi.org/10.17504/protocols.io.kqdg3qp9pv25/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 27, 2024
Last Modified: November 27, 2024
Protocol Integer ID: 113020
Abstract
Xenium protocols for FFPE tissue.
Tissue Prep for FFPE
Tissue Prep for FFPE
Follow the attached protocols in order.
Download Xenium In Situ for FFPE - Tissue Preparation Guide.pdfXenium In Situ for FFPE - Tissue Preparation Guide.pdf10.5MB

Download Xenium In Situ for FFPE Tissues – Deparaffinization & Decrosslinking.pdfXenium In Situ for FFPE Tissues – Deparaffinization & Decrosslinking.pdf9.3MB

Assay and Instrument
Assay and Instrument
Follow the attached protocols in order. These steps are the same for OCT samples
Download Xenium In Situ Gene Expression - Probe Hybridization, Ligation & Amplification.pdfXenium In Situ Gene Expression - Probe Hybridization, Ligation & Amplification.pdf8MB

Download Xenium Analyzer User Guide.pdfXenium Analyzer User Guide.pdf12.2MB