Jun 13, 2022

Public workspaceWorm Freezing Buffer

DOI
dx.doi.org/10.17504/protocols.io.14egnxjrzl5d/v1
  • 1Baylor College of Medicine
  • Adrien Assie: Uploader to Protocols.io, original protocol found on wormbook from Brener et al. 1974
  • Buck Samuel: Uploader to Protocols.io, original protocol found on wormbook from Brener et al. 1974
ycnz8k6.jpg
Icon indicating open access to content
QR code linking to this content
DOI: dx.doi.org/10.17504/protocols.io.14egnxjrzl5d/v1
External link: http://www.wormbook.org/chapters/www_strainmaintain/strainmaintain.html#d0e920
Protocol Citation: Adrien Assie, Buck Samuel 2022. Worm Freezing Buffer. protocols.io https://dx.doi.org/10.17504/protocols.io.14egnxjrzl5d/v1
Manuscript citation:
Brenner, S. (1974). Genetics 77, 71
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: April 10, 2019
Last Modified: June 13, 2022
Protocol Integer ID: 22275
Disclaimer
Original protocol found on wormbook from Brener et al. 1974

DISCLAIMER – FOR INFORMATIONAL PURPOSES ONLY; USE AT YOUR OWN RISK

The protocol content here is for informational purposes only and does not constitute legal, medical, clinical, or safety advice, or otherwise; content added to protocols.io is not peer reviewed and may not have undergone a formal approval of any kind. Information presented in this protocol should not substitute for independent professional judgment, advice, diagnosis, or treatment. Any action you take or refrain from taking using or relying upon the information presented here is strictly at your own risk. You agree that neither the Company nor any of the authors, contributors, administrators, or anyone else associated with protocols.io, can be held responsible for your use of the information contained in or linked to this protocol or any of our Sites/Apps and Services.
Abstract
Worm Freezing buffer recipe according to Brenner, S. (1974). Genetics77, 71. Found on wormbook see external link
Amount150 mL of Concentration100 % volume glycerol


Amount25 mL of Concentration1 Molarity (M) Potassium Phosphate Buffer, pH 6.0


Amount2.85 g of NaCl

Amount500 mL of M9 Buffer




Adjust deionized water toAmount1000 mL

Autoclave