License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: April 10, 2019
Last Modified: June 13, 2022
Protocol Integer ID: 22275
Disclaimer
Original protocol found on wormbook from Brener et al. 1974
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Abstract
Worm Freezing buffer recipe according to Brenner, S. (1974). Genetics77, 71. Found on wormbook see external link
150 mLof 100 % volume glycerol
25 mL of 1 Molarity (M) Potassium Phosphate Buffer, pH 6.0