Cells were stained with the following fluorochrome conjugated antibodies to assess polyfunctionality and memory:
Surface staining: CD8 V450 (clone RPA-T8, Cat 560347), CD3 V500 (clone SP34-2, Cat 560770), CD4 FITC (clone RPA-T4, Cat 555346 and clone M-T477, Cat 556615), CD45RA-PE (clone HI100, Cat 555489) all from BD Biosciences.
Intracellular staining: IFN-gamma APC (clone B27, BD Biosciences, Cat 554702), IL-2 PE (clone N7.48A, Beckman, Cat IM2718U) and TNF PCP-Cy5.5 (clone MAb11, eBioscience, Cat 45-7349-42).