A | B | |
50mM Tris HCl, PH 7.4 | 5mL (1M stock) | |
150mM NaCl | 5mL (3M stock) | |
1% Triton X-100 or NP-40 | 1 mL | |
0.1% SDS | 1mL (10% supply center solution) | |
1mM EDTA | 0.2 mL (0.5M stock) | |
10mM Naf | 0.042g (@L5P1 chemical room) | |
0.5% Sodium deoxylcholate | 0.5g (@L5P1 chemical room) | |
Add ddH2O to 100 mL | ||
Add PMSF [final] = 1mM and any other protease inhibitors immediately before use | ||
Add 1uL Pierce™ Universal Nuclease per 1mL |
RIPA buffer | 100mL | |
50mM Tris HCl, PH 7.4 | 5mL (1M stock) | |
150mM NaCl | 5mL (3M stock) | |
1% Triton X-100 or NP-40 | 1 mL | |
0.1% SDS | 1mL (10% supply center solution) | |
1mM EDTA | 0.2 mL (0.5M stock) | |
10mM Naf | 0.042g (@L5P1 chemical room) | |
0.5% Sodium deoxylcholate | 0.5g (@L5P1 chemical room) | |
Add ddH2O to 1000ml | ||
Add PMSF to a final concentration of 1mM and any other protease inhibitors immediately before use. | ||
Add 1 uL Pierce™ Universal Nuclease per 1mL |
4 x SDS sample buffer | For 1000ml | |
12% SDS | 120g | |
25% Glycerol | 250ml | |
150 mM Tris.HCl (pH 7.0 1M stock) | 150ml | |
0.03% Bromophenol Blue | 300mg | |
20% β-mercaptoethanol | 200ml | |
Add ddH2O to 50ml, aliquot and store at -20°C | ||
20% β-mercaptoethanol, (or 500 mM DTT replaced) should be added freshly before use. |