Sample loading buffer with DTT
Gel box with power source
Invitrogen 4-12% BisTris gel (10, 12, or 17-well)
iBlot 2 PVDF transfer pads
5% BSA or milk diluted in 1 x TBST
Primary antibody (protein target-dependent)
Secondary antibody + HRP (dependent on host species of the primary antibody)
ECL reagent (Classico, Cresendo, Forte)
iBright or comparable imaging device