May 08, 2024
WATER PRODUCTION FOR AWARE (Metals)
- 1IIM-CSIC
Protocol Citation: Antonio Cobelo 2024. WATER PRODUCTION FOR AWARE (Metals). protocols.io https://dx.doi.org/10.17504/protocols.io.36wgqn7yogk5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 08, 2024
Last Modified: May 08, 2024
Protocol Integer ID: 99418
Keywords: water sampling, water processing, water analysis, waste water treatment, advanced tertiary treatment SOP
Funders Acknowledgement:
Horizon Europe
Grant ID: 101084245
Abstract
The protocol summarises the procedures used for analytical control. The protocol describes the Standard Operating Procedure (SOP) for the optimization of advanced tertiary treatment of water, based on a comprehensive quality and risk assessment.
Guidelines
Directive 2008/105/EC of the European Parliament and of the Council of 16 December 2008 on environmental quality standards in the field of water policy.
Materials
| A | B | C | D | E | F | G | H | |
| Parameter | V (mL) x R | S | Processing | Analytical method | Result | LOD / LOQ | Goal value | |
| Metals | 50 x 2 | On ice | Acidification (0.15M HNO3) | ICPM-MS | Metals quantity (parts per billion (ppb) / parts per trillion (ppt) | Limit of Detection Al – 0.1 ppb V – 0.01 ppb Cr – 0.001 ppb Mn – 0.01 ppb Fe – 0.05 ppb Co – 0.001 ppb Ni – 0.01 ppb Cu – 0.01 ppb Zn – 0.05 ppb Th – 0.01 ppt Cd – 0.0005 ppb Csi – 0.005 ppb Ba – 0.01 ppb Pb – 0.005 ppb As – 0.01 ppb Sn – 1.0 ppt Sb – 1.0 ppt Pt – 0.1 ppt Tl – 0.01 ppt Bi – 0.01 ppt U – 0.01 ppt Ag – 0.01 ppt Hg – 0.01 ppt La – 0.01 ppt Ce – 0.01 ppt Pr – 0.001 ppt Nd – 0.01 ppt Sm – 0.005 ppt Eu – 0.005 ppt Gd – 0.01 ppt Tb – 0.005 ppt Dy – 0.005 ppt Ho – 0.005 ppt Er – 0.005 ppt Tm – 0.005 ppt Yb – 0.005 ppt Lu – 0.005 ppt |
Material: Metal-free centrifuge tubes
Safety warnings
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Metals
Metals
1d 21h
The water production for AWARE main activities includes three stages – disinfection by ultraviolet C radiation (UVC), storage for12:00:00 -24:00:00 (according to water load and season) and ozonation. The water quality is monitored at these three stages, for the parameters indicated in Figure 1 below.
1d 12h
Sampling, Processing, and Analyses
Water samples are collected (see Figure 2) and processed within a06:00:00 interval, before being shipped for the partner responsible for the analyses (Table 1). In case no processing is needed, samples are frozen and stored at-80 °C within03:00:00 .
For each sampling event, the date, day of the week and hour; the temperature and rain. Sampling points, indicated in Figure 2 were designated from A to I:
- Influent of primary treatment (A)
- Influent of biological treatment (activated sludge) (B)
- Treated secondary effluent (C)
- Sand filter effluent (D)
- UVC effluent (E)
- Storage for reuse tank effluent (F)
- Ozonation effluent (1 dose, e.g.,5 mg O3) - MITO3X technology - (G)
- Effluent of the vacuum UV oxidation (VUV) (H)
- Effluent of reactive storage / Influent of the recirculation aquaculture system (RAS) (I)
9h
Methods: The section below summarises the procedures used for analytical control – detailed protocols are annexed to this protocol.
Methods: The section below summarises the procedures used for analytical control – detailed protocols are annexed to this protocol.
12h
Metals:
Analysis: Detection and quantification of metals
Method: Acidified water samples were analyzed for several metals (e.g. Al, Cu, Ni, Co, Pb, Zn, Cd, Pt, Sb, Sn, U, As, Ag, Hg, REEs) by means of ICP-MS (7900 Agilent) using matrix-matched external calibration.
Observations: Samples were filtered/centrifuged within12:00:00 after collection, and then acidified to 0.15M HNO3.
12h
Parameters framed by Legal and Regulatory Requirements:
Parameters framed by Legal and Regulatory Requirements:
Using the EU Drinking Water Directive:
Metals - DIRECTIVE 2008/105/EC OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 16 December 2008 on environmental quality standards in the field of water policy
Protocol references
U.S. EPA. 1994. “Method 200.8: Determination of Trace Elements in Waters and Wastes by Inductively Coupled Plasma-Mass Spectrometry,” Revision 5.4. Cincinnati, OH.