Mar 25, 2022

Public workspaceWater filtration with Zymo Bashing Bead tubes and DNA/RNA Shield reagent for subsequent DNA and RNA extraction from microbial communities

DOI
dx.doi.org/10.17504/protocols.io.eq2lyn6zrvx9/v1
  • 1University of Guelph
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DOI: dx.doi.org/10.17504/protocols.io.eq2lyn6zrvx9/v1
Protocol Citation: Christopher A Hempel 2022. Water filtration with Zymo Bashing Bead tubes and DNA/RNA Shield reagent for subsequent DNA and RNA extraction from microbial communities. protocols.io https://dx.doi.org/10.17504/protocols.io.eq2lyn6zrvx9/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We used this protocol and it’s working.
Created: March 25, 2022
Last Modified: March 25, 2022
Protocol Integer ID: 59923
Keywords: Filtration, Zymo Bashing Bead tubes, DNA/RNA Shield reagent, DNA extraction, RNA extraction, Microbial community
Abstract
Filtration of water samples with Zymo Bashing Bead tubes and DNA/RNA Shield reagent for subsequent DNA and RNA extraction from microbial communities.
Materials
Zymo Bashing Bead tubes (ZR BashingBead Lysis Tubes (0.1 & 0.5 mm))
DNA/RNA Shield reagent
0.2 µm Nalgene Analytical Test Filter Funnels
Forceps, scissors, burner
Bleach + EtOH
Before start
Label bashing tubes (1 per sample + 1 filtration control) and load them with 1 mL of DNA/RNA Shield
Procedure
Procedure
Sterilize forceps and scissors with burner, then bleach, then EtOH before each filtration
Filter water sample, make sure to keep the filtration unit closed when opening the wrapping, to hold nothing but the sample over the unit, and to stand as far as possible away from the unit while it is open
Cut filter into small pieces (forceps and scissors) while filter lays on the filtration unit, make sure to stand as far as possible away from the unit
Transfer pieces into bashing tubes until all pieces are covered with DNA/RNA Shield
Clean filtration unit adapter with bleach after each filtration
Bashing tubes can be stored at -20°C