Aim: Reduction of any PCR inhibitors and potential concentration of RNA. Starting volume is 20 µl but this can be increased if further concentration is required (although for the latter tests on whether inhibitors are also concentrated have not been undertaken).
Notes: Use either AMPure RNA XP beads (Beckman Coulter Agencourt) or Mag-Bind® TotalPure NGS beads (Omega Bio-Tek), the latter being cheaper to use. Use aliquoted beads rather than directly from stock bottle to minimize risk of stock contamination. Ensure the aliquot is at room temperature and thoroughly mixed (vortexed) before use.
PCR Plates – Any style (skirted, non-skirted, full height, low profile), providing they fit the magnetic rack effectively.
Working environment – Ensure you work in a dust free environment, either a dust free bench or PCR cabinet. If you are in an area that may have a high risk for contamination, wipe down benches with household bleach (1 – 5%) prior to use.
Automation is possible, e.g. Opentrons (allowing at least 7 minutes on magnet).