Add 2 ml bleaching solution to the 2 ml solution of worms. Either vortex or vigorously shake for 5 minutes, checking worms every minute. Once bodies are dissolved add M9 to 14 ml, centrifuge at 1000 g for 60 sec and aspirate liquid. Note: after the first centrifugation a film can be seen over the eggs. We usually aspirate less liquid that time, leaving 1 ml, and reduce the volume left for the following centrifugations.