A | B | C | D | |
Primer set | Flanked region* | Amplicon | Covered mutations | |
Artic primers 76 Left 5’-AGGGCAAACTGGAAAGATTGCT-3’ 77 Right 5’-CAGCCCCTATTAAACAGCCTGC-3’ | 22819- 23500 | 725 bp | N439K, L452R/Q, Y453F, S477N, T478K, E484K/Q, S494P, N501Y/T/S, A570D, D614G | |
In house 1MS Fw 5’-TAACGCCACCAGATTTGCAT-3’ 2MS Rv 5’-ACACGCCAAGTAGGAGTAAGT-3’ | 22607- 23446 | 878 bp | K417T/N, N439K, L452R/Q, Y453F, S477N, T478K, E484K/Q, S494P, N501Y/T/S, A570D, D614G | |
A | B | C | D | |
Primer set | Flanked region* | Amplicon | Covered mutations | |
Artic primers 71 Left 5’- ACAAATCCAATTCAGTTGTCTTCCTATTC-3’ 73 Right 5’- CACCAGCTGTCCAACCTGAAGA-3’ | 21386-22324 | 989 bp | 𝚫69-70/144-145, 𝚫157-158, 𝚫241-243, S13I, L18F, T19R, T20N, P26S, Q52R, A67V, V70F, G75V, T76I, D80A, T95I, D138Y, W152C, E156G, R190S, D215G, A222V, W258L. | |