Create a result file in MultiQuant 3.0.3 by opening the software, selecting an LC-MS/MS data file acquired in Analyst software, that has been acquired following extraction using a protocol with carefully designed calibration curve and known calibration units. Select some or all of the samples in the batch of the file, selecting the processing method (as above) and allow the software to evaluate the data, before defining calibration ranges and assessing integration of peaks. To do this, follow these steps