Prepare ladder aliquots (upon first use of Nano 6000 kit):
a. Thaw RNA 6000 ladder tube at room temperature for 5 minutes.
b. Vortex and transfer contents (35 µl) into 0.65 ml tube.
c. Heat denature at 70°C for 2 minutes. Immediately transfer to ice bucket for five minutes.
d. Dispense 2.2 µl into 0.65 ml tubes with single-channel P20 repeater.
e. Label with date, as “Ladder, date”.
f. Store at -80°C until ready for use. One aliquot will supply two LabChips.