A | B | C | |
Time (min) | %B | Valve Position | |
0 | 5 | 10_1 | |
10 | 5 | 1_2 | |
13 | 15 | ||
70 | 45 | ||
72 | 95 | ||
76 | 95 | ||
80 | 5 | ||
90 | 5 |
A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | |
Time (min) | %B | Valve Position | |||||||||||||||||
0 | 5 | 10_1 | |||||||||||||||||
10 | 5 | 1_2 | |||||||||||||||||
13 | 15 | ||||||||||||||||||
70 | 50 | ||||||||||||||||||
72 | 95 | ||||||||||||||||||
76 | 95 | ||||||||||||||||||
80 | 5 | ||||||||||||||||||
90 | 5 |
A | B | |
High-High | ||
Spray voltage | 1800 | |
Sweep gas | 0 | |
Ion transfer tube temp | 320 | |
Application mode | Intact Protein | |
Pressure mode | Low Pressure | |
Advanced Peak Determination | True | |
Default charge state | 15 | |
S-lens RF | 30 | |
Source fragmentation | 15 eV |
A | B | |
High-High | ||
Detector type | Orbitrap | |
Resolving power | 120000 | |
m/z RP measured | 200 m/z | |
Scan range | 600-2000 | |
Mass range | Normal | |
AGC target | 1000000 | |
Normalized AGC target | 250% | |
Max Injection Time | 100 ms | |
Microscans | 4 | |
Data type | Profile | |
Polarity | Positive | |
Use wide quad isolation | True |
A | B | |
Intensity minimum | 20000 | |
Intensity maximum | 1E20 | |
Included charge states | 6-60 | |
Include undetermined charge states | False | |
Dynamic exclusion after n times | 1 | |
Dynamic exclusion duration | 60 s | |
Mass tolerance | 1.5 m/z | |
Exclude isotopes | True |
A | B | |
High-High | ||
Detector type | Orbitrap | |
Isolation mode | Quadrupole | |
Resolving power | 60000 | |
m/z RP measured | 200 m/z | |
Scan range | 350-2000 | |
AGC target | 1000000 | |
Normalized AGC target | 2000% | |
Max injection time | 400 ms | |
Microscans | 4 | |
Isolation window | 3 m/z | |
Activation type | HCD | |
Collision energy | 27 | |
Collision energy mode | Fixed | |
Polarity | Positive |