The mass list from LipidSearch is then exported and used in Compound Discoverer for improved alignment and quantitation. Mass tolerance, 10 ppm; minimum and maximum precursor mass, 0-5,000 Da; retention time limit, 0.1-30 min; Peak filter signal to noise ratio, 1.5; retention time alignment maximum shift, 1 min; minimum peak intensity, 10,000; compound detection signal to noise ratio, 3. Isotope and adduct settings were kept at default values. Gap filling and background filtering were performed by default settings. The MassList Search was customized with 5 ppm mass tolerance and 1 minute retention time tolerance. Area normalization was performed by constant median after blank exclusion.