Collection Kit Components:
PBS (Study team)
Media: DMEM + antibiotics (Study team)
Media tubes (x3 per collection) (Study team)
10% Formalin (Bionet)
FFPE cassette x 5 (Bionet)
Specimen container (Bionet)
OCT compound (Bionet)
OCT mold x 4 (Bionet)
OCT quick freeze box (Bionet)
Liquid nitrogen vapor (Bionet)
Cryovials x 3 (Bionet)
Collection kit containing 10 ml EDTA blood tubes x 2 (Bionet)
BLS will drop off PBS in specimen container at the start of the procedure.
Surgeon will excise 5-10 g visceral fat and place in specimen container with PBS. OR staff will page
BLS for specimen pickup.
The location of the tissue collection will be recorded by the surgeon.
BLS will pick up specimen immediately and request Bionet Research Specimen Order. Short
transit/processing time is critical.
See table below for processing priorities.
OCT and FF samples will be stored at -BOC until pick up.
Fresh in media samples will be stored at 4C until pick up.
BLS procurement will submit formalin specimens to BLS Histology for FFPE processing. Standard
fixation period of 24-72 hours. H&E requested.
Sample 1: FFPE, 1g
Sample 2: OCT, 1g
Sample 3: Flash Frozen, 1g
Sample 4: Fresh in Media, 2g
Sample 5: FFPE, 1g
Sample 6: OCT, 1g
Sample 7: Flash Frozen, 1g
Sample 8: Fresh in Media, Remainder
BLS will drop off PBS in specimen container prior to procedure.
Surgeon will excise 5 g subcutaneous fat and place in specimen container with PBS. OR staff will page
BLS for specimen pickup.
The location of the tissue collection will be recorded by the surgeon.
BLS will pick up specimen immediately and request Bionet Research Specimen Order. Short
transit/processing time is critical.
See table below for processing priorities.
OCT and FF samples will be stored at -BOC until pick up.
Fresh in media samples will be stored at 4C until pick up.
BLS procurement will submit formalin specimens to BLS Histology for FFPE processing. Standard
fixation period of 24-72 hours. H&E requested.
Sample 1: FFPE, 1g
Sample 2: OCT, 1g
Sample 3: Flash Frozen, 1g
Sample 4: Fresh in Media, 2g
Sample 5: FFPE, Remainder
Study team will set up blood processing instructions with TTL.
Study team will place order for blood in EPIC. Please provide BLS pager number in the
order notes for pickup contact.
Study team will provide preop staff with blood tube collection kits. 2 x 10 ml EDTA blood tubes will be in a bag labeled with BLS pager number for pick up contact.
Blood tubes will be held at room temperature until delivery to TTL or pick up by study team.
BLS will pick up blood tubes and submit to TTL for processing. If filled blood tubes are returned to BLS past TTL’s 2pm processing deadline, study team will be notified to pick up blood tubes and process through Laura Niederhofer’s lab.
Specimen Release: BioNet will email the research team when specimens are ready for pick-up. Specimens can be picked-up at the BioNet office before 4:00PM. Signature confirmation of
receipt will be required.
BLS will email study team for pickup ASAP since immediate pickup of fresh samples is ideal. Study team will pick up samples after 2pm.
Study team will pick up fresh, OCT, and snap frozen samples the same day.
If BLS was unable to submit blood to TTL, study team will pick up blood the same day.
Study team will pick up FFPE blocks when they are ready (~1-2 weeks after procedure day).
Follow-Up: If collection containers or media are stored with BioNet, the researcher will provide new ones for future collections when needed.