1. Tissue marking dyes black, red, yellow, Cancer Diagnostics, CAN MD2000
3. Fish gelatin, Sigma, G7041-100G
4. Poly-L-Lysine solution, 0.1%, Sigma, P8920
1. Workspace for GLP and BSL 2/2+
3. 18 Gauge needle BD Vacutainer™ Syringe Needle, Fisher, 23-021-020
5. Narrow Mouth Bio-Tite™ 40mL (1 oz.) 48mm Specimen Containers, Fisher 13-711-86
6. Peel-A-Way Embedding molds, 22x30mm, Polysciences, 18646B
7. Stereo Microscope, Nikon, SMZ 1000
8. Allis tissue forceps, Jarit 135-095
9. Bitran Freezer Bag, Fisher, 19240150
10. Cryostat, Leica, CM3050-S
11. Superfrost Plus Gold slides, Fisher, 15-188-48
12. Indium-Tin-Oxide (ITO) coated slides, Delta Technologies, CG-801N-S115
13. Vacuum packing unit, FoodSaver
14. Absorbent bench underpad, VWR, 56616-031
15. Dremel Rotary Tool, Dremel, 3000
16. Dremel Multi Vise, Dremel, 2500-1
17. Dremel Rotary Shield, Dremel, A550
18. Dremel 545 Diamond Wheel, Dremel, DCW0025
19. Dremel 402 mandrel, Dremel, 402
20. Zeiss Axio Z1, Zeiss, 430038-9001-000
21. Zeiss Colibri LED light source, Zeiss, 423052-9710-00
22. Optimal Cutting Temperature Polymer, Fisher SH75-125D
23. Foodsaver V2244 vacuum sealer
24. Food-grade vacuum freezer bags (many manufacturers)
1. 15% Fish Gelatin (made day of use)
Warm 100 mL of milliq H2O
Gently mix until gelatin is completely dissolved
2. Poly-L-Lysine Coating Slides
Dilute the poly-L-lysine solution 1:10 in water and dilute 1:10 again
Place ITO slides in diluted poly-L-lysine solution for 5 minutes
Remove slides and allow to air dry
Place ITO slides in diluted solution again, for 5 minutes
Use an air line to blow off access solution
Place in 37oC oven to completely dry