| Product Name | Manufacturer | Pro. # | Notes: |
| CellEvent Senescence Green Flow
Cytometry Assay Kit | TF | C10841 (200 assays) | Optimal Conditions: -Fix in 2% paraformaldehyde -10 min fix at RT in dark -1:750 dilution -90 minute incubation at 37C without
CO2 in dark |
| TrueNuclear Transcription Factor
Buffer Set | Biolegend | 424401 (120 tests) | Per protocol for 96-well plates, with
60-min fixation and 45 minute staining, all at RT in dark |
| Human TruStain FCX | Biolegend | 422302 (200 tests) | During surface staining |
| Cell Staining Buffer | Biolegend | 420201 (500 mL) | Can substitute FACSwash (PBS without
Mg/Ca + 2% HI-FBS and 1 mM EDTA) |
| Fixative Solution | Biolegend | 420801 (100 mL) | Used prior for fixation during β-gal activity portion of protocol |
| FluoroFix Buffer | Biolegend | 422101 (200 tests) | Used for flow cytometry acquisition |
| Spectroflow QC Beads (lot 2003) | Cytek | B7-10001 | Used for daily Cytek Aurora QC. |
| Brilliant Stain Buffer Plus | BD | 566385 | Used in all staining tubes during
surface and ICS. |
| CEF Pool Extended (MHC I) | JPT peptide | PM-CEF-E-1 | Stored in 20-test aliquots at a
concentration of 0.5 mg/mL), requiring 0.45 uL per 225 uL stimulation. |
| CEFX Ultra SuperStim Pool (MHC II) | JPT peptide | PM-CEFX-3 | Stored in 20-test aliquots at a
concentration of 0.5 mg/mL), requiring 0.45 uL per 225 uL stimulation. |
| BD CompBeads Anti-Mouse | BD | 552843 | We use single-cell stained controls
for compensation/unmixing, however we run comp beads as well as cells for
troubleshooting issues. |
| BD CompBeads Ant-Rat/Hampster | BD | 552845 | See above comment |
| REA control antibody, human IgG1, PE,
Reaffinity | Miltenyi | 130-118-347 (100 tests) | Used for FMO controls prior to batch
runs |
| BV421 Mouse IgG1 k isotype control | Biolegend | 400158 | Used for FMO controls prior to batch
runs |
| AF647 Mouse IgG2b k isotype control | Biolegend | 400330 | Used for FMO controls prior to batch
runs |
| PE-Cy7 Mouse IgG2b k isotype control | TF | 25-4732-81 | Used for FMO controls prior to batch
runs |
| PerCP-efluor710 Mouse IgG2b k isotype
control | TF | 46-4732-82 | Used for FMO controls prior to batch
runs |
| PE-CF594 Mouse IgG1 k isotype control | BD | 562292 | Used for FMO controls prior to batch
runs |
| Corning Costar 96-well PS U-bottom
tissue culture-treated plates | Corning | 3799 | Plates for staining |
| Corning Costar 96-well PP Cluster
tubes (1.2 mL) | Corning | 4401 | Cluster tubes for acquisition |