Aug 15, 2023

Public workspaceU54 SCENT Olink® Target 96

DOI
dx.doi.org/10.17504/protocols.io.j8nlkookwv5r/v1
  • 1Division of Medical Oncology, Department of Medicine, Duke University School of Medicine, Durham, NC, USA.
Valerie Bekker
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DOI: dx.doi.org/10.17504/protocols.io.j8nlkookwv5r/v1
Protocol Citation: andrew.nixon, Chris Brady 2023. U54 SCENT Olink® Target 96. protocols.io https://dx.doi.org/10.17504/protocols.io.j8nlkookwv5r/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 15, 2023
Last Modified: August 15, 2023
Protocol Integer ID: 86501
Abstract
This protocol outlines the sample preparation and running of the Olink 96 panel
Preparation
Preparation
Aliquoting and Day of Preparation

For Aliquoting of EDTA plasma samples:
  • Thaw all EDTA plasma samples in a -4°C refrigerator until ready to use.
  • Keep Samples in a chilled rack whenever possible.
  • Match your parent tubes (Original tubes shipped) with a prelabelled 2mL clarification tube.
  • Vortex all samples on a Vortex Genie with a vortex setting of 7-10.
  • Transfer samples from the parent tube to the clarification tube.
  • In a pre-cooled centrifuge set to -4°C centrifuge all samples for 5 minutes on a setting of 20,000 rcf.
  • Carefully remove samples from centrifuge into a chilled rack.
  • Transfer liquid from the clarification tube to a prelabelled aliquot tube in desired amounts.
  • Using Liquid Nitrogen, freeze back the tubes and place in a -80°C freezer until ready to use.
For EDTA samples on day of use:
  • Thaw all EDTA plasma samples in a -4°C refrigerator until ready to use.
  • Keep Samples in a chilled rack whenever possible.
  • Vortex all samples on a Vortex Genie with a vortex setting of 7-10.
  • In a pre-cooled centrifuge set to -4°C centrifuge all samples for 5 minutes on a setting of 20,000 rcf.
  • Return the samples to a chilled rack.
  • Your samples are ready for use.
Running Olink Panel
Running Olink Panel
Once samples are prepared use the following links to access the protocols: