Jun 12, 2023
U54 SCENT 10X Genomics Visium Protocol
- Karen Abramson1,
- Simon Gregory1,2
- 1Duke Molecular Physiology Institute, Durham, NC, USA;
- 2Department of Neurology, Duke University, Durham, NC, USA
Protocol Citation: Karen Abramson, Simon Gregory 2023. U54 SCENT 10X Genomics Visium Protocol . protocols.io https://dx.doi.org/10.17504/protocols.io.6qpvr3xozvmk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 07, 2023
Last Modified: June 12, 2023
Protocol Integer ID: 83010
Abstract
This document outlines the 10X Genomics Visium Protocol used for normal lung and colon tissues for SCENT at Duke University.
10X Genomics Visium Protocol
10X Genomics Visium Protocol
Once slides received, the following 10x Genomics protocol is followed:
10x Genomics kit is used – Visium (v1) Spatial for FFPE Gene Expression Kit, Human Transcriptome.
Libraries sequenced on Illumina sequencers to a read depth of a least 25k reads/spot, with read lengths of 28x10x10x50.