A | B | |
1x TNE (TNE) + Inhibitors | 1 mL | |
2x TNE | 500 L | |
100x HALT inhibitor | 10 µL | |
100x HALT EDTA | 10 µL | |
H2O to final | 480 µL |
A | B | |
Complete 2x TNE (2x CTNE) + Inhibitors | 1 mL | |
2x TNE | 500 µL | |
1% SDS | 100 µL | |
0.5% NP-40 | 100 µL | |
0.5% DOC | 100 µL | |
100x HALT inhibitor | 10 µL | |
100x HALT EDTA | 10 µL | |
H2O to final | 180 µL |