Apr 04, 2024
Two-action sequence reinforcement
Forked from Single action sequence reinforcement
- Jonathan Tang1,2,3
- 1Department of Neuroscience, Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY 10027, USA;
- 2Seattle Children’s Research Institute, Center for Integrative Brain Research, Seattle, WA, USA;
- 3University of Washington School of Medicine, Department of Pediatrics, Seattle, WA, USA
Protocol Citation: Jonathan Tang 2024. Two-action sequence reinforcement . protocols.io https://dx.doi.org/10.17504/protocols.io.3byl49248go5/v1
Manuscript citation:
Tang, J.C.Y., Paixao, V., Carvalho, F. et al. Dynamic behaviour restructuring mediates dopamine-dependent credit assignment. Nature (2023). https://doi.org/10.1038/s41586-023-06941-5
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: April 04, 2024
Last Modified: May 31, 2024
Protocol Integer ID: 97817
Keywords: ASAPCRN, mouse, behavior, brain
Funders Acknowledgement:
Life Sciences Research Fellowship
NINDS K99/R00 Award
Grant ID: 1K99NS112575
NIH
Grant ID: 5U19NS104649
Aligning Science Across Parkinson’s (ASAP)
Grant ID: ASAP-020551
Disclaimer
The protocols.io team notes that research involving animals and humans must be conducted according to internationally-accepted standards and should always have prior approval from an Institutional Ethics Committee or Board.
Abstract
Two-action sequence reinforcement protocol for mouse studies from Tang et al 2023.
Guidelines
Individual mice were subjected to a single session of protocol each day, with sessions following each other on consecutive days.
Materials
The open-field box is made of 410 × 400 mm grey opaque acrylic walls and a 410 × 400 mm white matte acrylic base.
First reinforcement session+ Baseline
First reinforcement session+ Baseline
Mice were placed in a grey open-field behaviour recording for this protocol.
To acquire baseline behavior, individual mice were allowed to behave freely inside the box for 30 minutes when the laser stimulation was not available for reinforcement.
After initial behavior acquisition, 60 minutes of laser availability was given. During the
reinforcement periods, when the closed-loop system detects performance of the
proximal action (T1) of interest, the algorithm enters a state in which the
laser is triggered on performance of the distal action (T2), regardless of the
amount of time that has elapsed between the latest T1 and T2.
Remaining Sessions
Remaining Sessions
90 minutes of closed loop reinforcement were made available for individual mice during subsequent sessions.
During the reinforcement periods, when the closed-loop system detects performance of the
proximal action (T1) of interest, the algorithm enters a state in which the
laser is triggered on performance of the distal action (T2), regardless of the
amount of time that has elapsed between the latest T1 and T2.
Extinction protocol
Extinction protocol
An extinction protocol was carried out comprising of 20-minute maintenance of reinforced behavior with laser availability, followed by 60 minutes of extinction of reinforced behavior without laser availability, followed by 20-minute re-acquisition of reinforced behavior with laser availability.