Jun 01, 2023

Public workspaceTween 60-esculin Agar (TE slant)

DOI
dx.doi.org/10.17504/protocols.io.yxmvm73e5v3p/v1
  • 1Duke University
Amy Gladfelter
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DOI: dx.doi.org/10.17504/protocols.io.yxmvm73e5v3p/v1
Protocol Citation: Amy Gladfelter 2023. Tween 60-esculin Agar (TE slant). protocols.io https://dx.doi.org/10.17504/protocols.io.yxmvm73e5v3p/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 03, 2018
Last Modified: June 01, 2023
Protocol Integer ID: 17460
Abstract
This media is used to determine ability of Malassezia species to hydrolyzeesculin and utilize Tween 60.
Attachments
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Malassezia media_1.p...
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Guidelines
This media is used to determine ability of Malassezia species to hydrolyze esculin and utilize Tween 60.
peptone Amount10 g

glucose Amount10 g

yeast extract Amount2 g

ferric ammonium citrate Amount0.5 g

esculin Amount1 g

agar Amount15 g

Tween 60 Amount5 mL

dH2O Amount1 L

Sterilize by autoclaving.