Name | Catalog # | Vendor | |
GentleMACS C Tubes | 130-093-237 | Miltenyi B | |
Tween-20 | P-7949 | Sigma-Aldrich | |
DNAse/RNAse Free Distilled Water | 10977023 | Thermo Fisher Scientific | |
MACS BSA Stock Solution (10%) | 130091376 | Miltenyi Biotec | |
Magnesium Chloride Solution for Molecular Biology (1.00 M) | M1028 | Sigma-Aldrich | |
Falcon‱ Cell Strainers - Mesh size: 40um; blue | 102095-532 | VWR | |
5M NaCl Solution | AM9759 | Thermo Fisher Scientific | |
UltraPure 1M Tris-HCI Buffer pH 7.5 | 15567027 | Thermo Fisher Scientific | |
Calcium Chloride 1M Sterile | 97062-820 | VWR | |
INCYTO C-Chip Neubauer Improved Disposable Hemocytometers | 22-600-100 | VWR International Ltd | |
Falcon‱ 15 mL Conical Centrifuge Tubes | 05-527-90 | Fisher Scientific | |
Falcon‱ 50 mL Conical Centrifuge Tubes | 14-432-22 | Fisher Scientific | |
Falcon‱ Round-Bottom Polystyrene Test Tubes with Cell Strainer Snap Cap | 08-771-23 | Fisher Scientific | |
Protector RNase Inhibitor (40 U/μL) | RNAINH-RO | Roche Diagnostics | |
PBS (1X, pH 7.4) without Calcium and Magnesium | 10010023 | Fisher Scientific |
Reagent | Stock Concentration | 2X ST Buffer Concentration | Volume for 50 mL of 2X ST Buffer | |
NaCl | 5 M | 292 mM | 2.92 mL | |
Tris | 1 M | 20 mM | 1 mL | |
CaCl2 | 1 M | 2 mM | 100 μL | |
MgCl2 | 1 M | 42 mM | 2.1 mL | |
H2O | - | - | 43.88 mL |
Reagent | Volume for 2 mL Working Solution (per Sample) | |
2x ST stock solution | 1000 μL | |
MACS BSA Stock Solution (10%) | 2 μL (0.01% final concentration) | |
10% Tween-20* | 6 μL | |
H2O | 942 μL | |
Protector RNase Inhibitor (40 U/μL) | 50 μL (1 U/μL final concentration) |
Reagent | Volume for 3.5 mL Working Solution (per Sample) | |
2x ST stock solution | 1748 μL | |
H2O | 1664.5 μL | |
Protector RNase Inhibitor (40 U/μL) | 87.5 μL |
Reagent | Volume for 3.5 mL Working Solution (per Sample) | |
PBS 1X | 875 μL | |
MACS BSA Stock Solution (10%) | 100 μL | |
Protector RNase Inhibitor (40 U/μL) | 25 μL |