May 11, 2018

Public workspaceTRIS buffer

DOI
dx.doi.org/10.17504/protocols.io.p2vdqe6
  • 1University of Edinburgh
  • Vale Lab
Katy M Monteith
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DOI: dx.doi.org/10.17504/protocols.io.p2vdqe6
Protocol Citation: Katy M Monteith 2018. TRIS buffer. protocols.io https://dx.doi.org/10.17504/protocols.io.p2vdqe6
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 11, 2018
Last Modified: May 11, 2018
Protocol Integer ID: 12085
Keywords: TRIS buffer, TRIS-HCl
Abstract
This protocol describes how to make TRIS buffer (TRIS-HCl) at a 1M concentration and a pH 8.0
Guidelines
This protocol describes how to achieve a TRIS buffer at a pH 8.0. If a lower pH is required, i.e. pH 7.4, then add more hydrochloric acid until desired pH is reached
Materials
MATERIALS
ReagentTris Base, Molecular Biology Grade, 2,500gPromegaCatalog #H5135
ReagentHydrochloric acidSigma AldrichCatalog #H1758
Safety warnings
Read SDS for TRIS base and Hydrochloric acid prior to use.
Add 121.14g of TRIS base to 800 mL of triple distilled H₂O
Stir solution on magnetic plate using magnetic flea until TRIS base completely dissolved
Using a pH meter, adjust pH of solution to pH 8.0 by adding concentrated hydrochloric acid (HCl)
Add triple distilled H₂O to bring final volume of solution to 1 L
Autoclave