Jun 18, 2024
Transgenic mouse colony maintenance
- Ariadna Laguna1,
- Iria Carballo-Carbajal1,
- Miquel Vila2
- 1vall d'hebron research institute;
- 2VHIR-CIBERNED-ASAP
Protocol Citation: Ariadna Laguna, Iria Carballo-Carbajal, Miquel Vila 2024. Transgenic mouse colony maintenance. protocols.io https://dx.doi.org/10.17504/protocols.io.yxmvmeyx5g3p/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 18, 2024
Last Modified: June 18, 2024
Protocol Integer ID: 102012
Funders Acknowledgement:
Ministry of Science and Innovation
Grant ID: PID2020-116339RB-I00
Abstract
Methods for the generation and maintenance of transgenic tyrosinase mice
Pronuclear microinjection of the human
tyrosinase complementary DNA fused to the rat tyrosine hydroxylase promoter into C57Bl6-SJL (RRID:MGI:5656718) mouse zygotes
Selection of positive lines and backcrossing for 8-10 generations using
C57BL/6J mice (Charles River; RRID:MGI:3028467)
Maintainance in heterozygosis by crossing heterozygous males with C57BL76J females
Housed two to five per cage with ad libitum
access to food and water during a 12 hours (h) light/dark cycle (light-on 8
a.m.)