To spread in large plates (150 mm) PDA.
1.- PDA with hyg and Carb (4 ml Hyg and 1 ml Carb per L): dispense 50 ml.
2.- PDA with Carb (1 ml per Liter): dispense 13.5 ml.
3.- Conidia transformants: 1 ml
4.- Low-melt PDA with Carb (1 ml per L). 13.5 ml. 37 C. we can mix with conidia.
5.- PDA layer carb and hyg. 13.5 ml, kept at 55 C.