1. Human Transferrin conjugated with Alexa Fluor 647 (Thermo Fisher, Catalog no: T23366).
Note: Transferrin conjugate is also available with Alexa Fluor 488, Alexa Fluor 546. The user may want to use a specific conjugate based on the need of their experiments. We have optimized this protocol for Transferrin conjugated with Alexa Fluor 647.
2. Basal Neuronal media- No added supplements like (N2, B27, NEAA, Glutamax and growth factors).
Note: The user may use their desired neuronal medium, but they have to make sure that the media does not contain any Transferrin.