1. Clean the working area and all equipment: wipe down with 10% bleach and let dry. Wipe down with 70% ethanol and let dry. Then, wipe down using RNase AWAY and let dry.
2. Prepare the 6 purification plates:
- Sample plate: Add 400μL lysate, 25 μL NucleoMag®B-Beads (vortex the bottle at max speed before use), and 475 μL MWA2 into each well required for purification.
- Wash 1 plate: 850 μL MWA3 to each well required for purification.
- Wash 2 plate: (same as plate Wash 1): 850 μL MWA3 to each well required for purification.
- Wash 3 plate: Add 850 μL MWA4 to each well required for purification.
- Elution plate: Add 100 μL RNase-free H2O to each well required for purification.
- Tip plate: Place KingFisher 96-tip comb into an empty KingFisher 96-well plate. While opening the 96-tip comb plate, pay attention to not touch the tips.