A | B | C | |
Items description | Catalogue number | Suppliers/Manufacturers | |
Ammonium Acetate 7.5M Solution | A2706-100ML | Sigma Aldrich | |
Chloroform:Isoamyl alcohol (24:1) | ACR327155000 | Thermo Fisher | |
CTAB | 52365-50G | Sigma Aldrich | |
Distilled water Ultra-Pure | 10977015 | Thermo Fisher Scientific | |
DNA LoBind tubes 1.5ml | 30108051 | Eppendorf | |
Dry ice | - | - | |
Dynabeads M-270 Carboxylic Acid | 14306D | Thermo Fisher | |
EDTA (0.5M), pH-8, Nuclease-free | AM9260G | Life Technologies | |
Ethanol (>98%) | US015017 | Thermo Fisher Scientific | |
Falcon tube 15ml | FAL352096 | In Vitro Technologies | |
Liquid Nitrogen (LN2) | - | - | |
P1000 wide bore pipette tips | 2079GPK | Thermo Fisher Scientific | |
P200 wide bore pipette tips | LC1152-965 | Adelab Scientific | |
PEG 8000 | V3011 | Promega | |
Proteinase K (PK) Solution | MC5005 | Promega | |
Qubit 1× dsDNA HS Assay Kit | Q33231 | Life Technologies | |
RNase solution | A7973 | Promega | |
Sodium Chloride | 71580-500G | Sigma Aldrich | |
UltraPure 1M Tris-HCI, pH-8 | 15568025 | Life Technologies | |
β-mercaptoethanol | M6250-100 mL | Sigma Aldrich |
A | B | |
Tris-HCl | 100 mM | |
EDTA | 20 mM | |
CTAB (w/v) | 4% | |
NaCl | 1.4 M | |
PVP 360k (w/v) | 1% | |
β-mercaptoethanol (add just before use)] | 2% |
A | B | |
1M Tris-HCl (pH = 8) | 5 ml | |
0.5M EDTA (pH = 8) | 2 ml | |
CTAB powder | 2 g | |
PVP | 0.5 g | |
NaCl | 4 g |
A | B | |
Tris-HCl | 100 mM | |
EDTA | 20 mM | |
CTAB (w/v) | 2% |
A | B | |
1M Tris-HCl (pH = 8) | 5 ml | |
0.5M EDTA (pH = 8) | 2 ml | |
CTAB powder | 1 g |
A | B | |
EDTA | 2 mM | |
Tris-HCl | 10 mM | |
NaCl | 1 M |
A | B | |
NaCl | 581 mg | |
0.5M EDTA (pH=8) | 40 μl | |
1M Tris-HCl (pH=8) | 100 μl |
A | B | |
Dynabeads™ M-270 Carboxylic Acid | 4% | |
PEG8000 | 18% | |
NaCl | 1 M | |
Tris-HCl pH-8 | 10 mM | |
EDTA pH-8 | 1 mM |