Use Ion Source Settings from Tune = Checked
Method Duration (min)= 100
Infusion Mode (LC)= False
FAIMS Mode = Not Installed
Application Mode = Peptide
Advanced Peak Determination = True
Desired minimum points across the peak = 6
Use Wide Quad Isolation = True
Orbitrap Resolution = 120K
Scan Range (m/z) = 500-2000
Maximum Injection Time (ms) = 200
Normalized AGC Target = 250%
Maximum Injection Time Type = Custom
Use ETD Internal Calibration = False
Source Fragmentation = False
Enhanced Resolution Mode = Off
Include charge state(s) = 5-35
Include undetermined charge states = False
Filter IntensityThreshold
Maximum Intensity = 1E+20
Minimum Intensity = 20000
Relative Intensity Threshold = 20
Intensity Filter Type = IntensityThreshold
Exclude after n times = 1
Exclusion duration (s) = 30
Use Common Settings = False
Perform dependent scan on single charge state per precursor only = True
Data Dependent Properties
Data Dependent Mode= Number of Scans
Number of Dependent Scans= 5
Desired minimum points across the peak = 6
Isolation Mode = Quadrupole
Enable Intelligent Product Acquisition for MS2 Isolation = False
Reported Mass = Original Mass
Multi-notch Isolation = False
Use calibrated charge dependent ETD parameters = False
ETD Reaction Time (ms) = 15
ETD Reagent Target = 700000
Max ETD Reagent Injection Time (ms) = 200
ETD Supplemental Activation = False
Orbitrap Resolution = 60K
Maximum Injection Time (ms) = 250
Inject ions for all available parallelizable time = False
Normalized AGC Target = 1000%
Maximum Injection Time Type = Custom
Use ETD Internal Calibration = False
Source Fragmentation = False
Enhanced Resolution Mode = Off