Use Ion Source Settings from Tune = Checked
Method Duration (min)= 90
Infusion Mode (LC)= False
FAIMS Mode = Not Installed
Application Mode = Intact Protein
Pressure Mode = Low Pressure
Default Charge State = 10
Advanced Peak Determination = True
Desired minimum points across the peak = 6
Use Wide Quad Isolation = True
Orbitrap Resolution = 120K
Scan Range (m/z) = 500-2000
Maximum Injection Time (ms) = 400
Normalized AGC Target = 250%
Maximum Injection Time Type = Custom
Use ETD Internal Calibration = False
Source Fragmentation = True
Enhanced Resolution Mode = Off
Include charge state(s) = 4-35
Include undetermined charge states = False
Exclude after n times = 1
Exclusion duration (s) = 30
Use Common Settings = False
Perform dependent scan on single charge state per precursor only = True
Data Dependent Properties
Data Dependent Mode= Number of Scans
Number of Dependent Scans= 4
Desired minimum points across the peak = 6
Isolation Mode = Quadrupole
Enable Intelligent Product Acquisition for MS2 Isolation = False
Reported Mass = Original Mass
Multi-notch Isolation = False
Scan Range Mode = Define m/z range
Collision Energy Mode = Fixed
Collision Energy (%) = 35
Activation Time (ms) = 10
Multistage Activation = False
Orbitrap Resolution = 60K
Scan Range (m/z) = 400-2000
Maximum Injection Time (ms) = 200
Inject ions for all available parallelizable time = False
Normalized AGC Target = 1000%
Maximum Injection Time Type = Custom
Use ETD Internal Calibration = False
Source Fragmentation = False
Enhanced Resolution Mode = Off