Nov 29, 2023

Public workspaceTop agar: 0.7% w/v LB(Miller) agar

  • 1macquarie uni, jaschke labs
Open access
Protocol CitationHANNAH ZHU 2023. Top agar: 0.7% w/v LB(Miller) agar. protocols.io https://dx.doi.org/10.17504/protocols.io.eq2lyj2oelx9/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 28, 2023
Last Modified: November 29, 2023
Protocol Integer ID: 91547
Abstract
how to make Top agar for plaque assay experiment
Materials
Tryptone
Yeast extract
NaCl
Agar
Top agar: 0.7% w/v LB(Miller) agar
Top agar: 0.7% w/v LB(Miller) agar
15m
Dissolve following components in 200 mL MilliQ water

componentsfinal concentrationfor 200mls
Tryptone1% w/v2 g
Yeast extract0.5% w/v1 g
NaCl1% w/v2 g
Agar0.7%1.4 g


Autoclave at Temperature121 °C for Duration00:15:00

15m
Store in oven (> Temperature50 °C )