Feb 14, 2025
Version 1
Tissue Fixation - 10% formalin samples V.1
- Roy Ambli Dalmo1
- 1The Arctic University of Norway
Protocol Citation: Roy Ambli Dalmo 2025. Tissue Fixation - 10% formalin samples. protocols.io https://dx.doi.org/10.17504/protocols.io.8epv52konv1b/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 13, 2025
Last Modified: February 14, 2025
Protocol Integer ID: 120177
Abstract
Tissue Fixation - 10% formalin samples
Protocol tissue fixation - 10% formalin
Protocol tissue fixation - 10% formalin
Cut tissue to proper size.
The size can be 2x2 mm to 1x2 cm but thickness should be 3mm for better fixation.
The cutting surface of the tissue should be flat and smooth.
Transfer tissue to fixative and swirl the container to ensure all tissues pieces are completely immersed in fixative.
The volume of fixative must be 20-30 times the tissue volume
Fix tissue at 20˚C for overnight.
Transfer the fixed tissue pieces to 96% ethanol and freeze (-20°C)
Note
Samples to be fixed:
1) Old scar tissue (skin) from seal lice infestation,
2) Fresh/newly formed scar form sea lice infestation
3) Normal non-scarred tissue.
2-5 pieces from each fish.
In total: 3-10 fish (you see what you get).