The methods included here are in support of research submissions from the www.OrsburnLab.org group in 2023 from a TIMSTOF Flex system with EasyNLC1200, EvoSep One and MALDI (1) front end sources.
pasefRIQ used for SCoPE-MS (DefaultTIMSTOF40_TMT_highsensitivity_pasefSCOPE
diaPASEF methods optimized for the EvoSep One - diaPASEF-shortgradient-1800V
the same - optimized for EasyNLC 1200 using a 10um emitter - TIMSTOF_40_shortgradient_DIA
ddaPASEF method for normal high input (100ng - 500ng proteomics loads) -DefaultDDA_TIMST40_5_6_2023
ddaPASEF methods optimized for the EvoSep One Whisper for ultralow input immunoenriched samples - DefaultDDA_TIMS40_high_sensitivity
A MALDI-MS method for TIMSTOF Flex to test the effectiveness of direct transfer of MALDI instrument methods between different TIMSTOF imaging mass spectrometers:
20220925_SCZ_GelMethod_AngelLab_transfer.m