Nov 02, 2023
- 1University of Cambridge
Protocol Citation: Alexander Röntgen 2023. TEM. protocols.io https://dx.doi.org/10.17504/protocols.io.q26g7pd79gwz/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 01, 2023
Last Modified: November 02, 2023
Protocol Integer ID: 90279
Abstract
TEM protocol.
Preparation
Preparation
Recover samples by pipetting out of aggregation plate
Glow-discharge grids (3-mm 300-mesh (EM Resolutions Ltd))
Sample spotting and staining
Sample spotting and staining
Take up grid with tweezers
Spot 2.3 µL sample on grid
Stain sample with 2.3 µL 2% uranyl acetate for 40 s at room temperature
Imaging
Imaging
Image grids on an electron microscope (FEI Tecnai G2 transmission electron microscope)