Rehydrate the samples at RT on a shaker with a falling Methanol gradient.
In the meantime, start preparing the other solutions:
20 g Triton-X 100 for a final concentration of 0.2% (W/V)
(drip from a cut-off bulb pipette into the flask while weighing it on a scale, as Triton is very viscous an therefore difficult to pipet reliably).
Permeabilization solution (for 100 ml)
Sodium Azide (from stock solution) for a final concentration of 0.005% (w/v)
Stir, then fill to 100 ml with PTx.2
Blocking Buffer (for 100 ml)
6 ml Neutral Donkey Serum
Sodium Azide (from stock solution) for a final concentration of 0.005% (w/v)
Stir, then fill to 100 ml with PTx.2
Heparin from stock for a final concentration of 0.01 g/L
Sodium Azide (from stock solution) for a final concentration of 0.005% (w/v)
Stir, then fill to 100 ml with PTx.2