Component | Final concentration | Amount | |
RPMI 1640 | n/a | To 500 mL | |
L-glutamine | 1x | 5 mL | |
Penicillin-streptomycin solution (10×) | 1× | 5 mL | |
2-mercaptoethanol (1 M) | 50 μM | 25 μL | |
FBS | 10% (v/v) | 50 mL | |
HEPES 1M | 25mM | 12.5 mL | |
sodium pyruvate | 1x | 5 mL | |
MEM non-essential amino acids | 1x | 5 mL |
Reagent | Quantity (for 1000 mL) | Final concentration | |
NH4Cl | 8.02 g | 150 mM | |
KHCO3 | 1 g | 10 mM | |
Na2EDTA | 37.2 mg | 0.1 mM |
1 | 2 | 3 | 4 | 5 | 6 | |
A | ||||||
B | 1 | 7 | 13 | 19 | PMA+ion | |
C | 2 | 8 | 14 | 20 | No peptide | |
D | 3 | 9 | 15 | 21 | No peptide | |
E | 4 | 10 | 16 | 22 | PMA+ion | |
F | 5 | 11 | 17 | 23 | No peptide | |
G | 6 | 12 | 18 | 24 | No peptide | |
H |
1 | 2 | 3 | 4 | 5 | 6 | |
A | ||||||
B | 1 | 7 | 13 | 19 | ||
C | 2 | 8 | 14 | 20 | ||
D | 3 | 9 | 15 | 21 | ||
E | 4 | 10 | 16 | 22 | ||
F | 5 | 11 | 17 | 23 | ||
G | 6 | 12 | 18 | 24 | ||
H |