This protocol describes the three surgical procedures required for simultaneous optogenetic stimulation in the Substantia Nigra pars compacta (SNc) and acute fiber photometry in the dorsal striatum:
1. Viral injection of a red-shifted channelrhodopsin, ChRmine, in the SNc, and a green dopamine sensor, GRAB-DA3m, in the dorsal striatum.
2. Fiber-optic cannula implantation into the SNc for optogenetic stimulation.
3. Craniotomy above the dorsal striatum for acute fiber photometry.