Apr 11, 2022
Version 2
Suggested protocol for loading a DNA Ladder/marker V.2
- 1New England Biolabs
External link: https://www.neb.com/protocols/2018/08/03/suggested-loading-protocol-for-dna-ladders-and-markers
Protocol Citation: New England Biolabs 2022. Suggested protocol for loading a DNA Ladder/marker. protocols.io https://dx.doi.org/10.17504/protocols.io.zkxygx6zg8j2/v2Version created by New England Biolabs
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: May 10, 2020
Last Modified: April 11, 2022
Protocol Integer ID: 36824
Keywords: agarose gel, how to load a gel, loading a ladder, loading a marker
Abstract
This is the suggested protocol for use with:
Materials
MATERIALS
Gel Loading Dye, Purple (6X), no SDS - 4.0 mlNew England BiolabsCatalog #B7025S
Safety warnings
Please refer to the Safety Data Sheets (SDS) for health and environmental hazards.
Before start
This protocol is recommended for a 5 mm wide gel lane. The components of the mixture should be scaled up or down, depending on the width of the lane.
Prepare loading mixture (6 μl total volume):
Note
Dilute only 1 µl of DNA Ladder at a time.
| A | B | |
| Distilled water (dH20)* or TE Buffer | 4 μl | |
| Gel Loading Dye, Purple (6X), no SDS | 1 μl | |
| DNA Ladder/Marker | 1 μl | |
| Total Volume | 6 μl |
*For multiple loads, dilution, and storage, use TE or other buffer of minimal ionic strength instead of water. DNA may denature if diluted and stored in dH20.
Mix gently by pipetting.
Load onto the agarose gel.