Reagent/consumable | Supplier | Catalogue number | |
Reagents | |||
Water | Sigma Aldrich/Merck | 1153332500 | |
20x SSC buffer | Thermo Fisher Scientific | AM9770 | |
20% SDS solution | Thermo Fisher Scientific | AM9820 | |
Consumables | |||
Square media bottle 500 ml | VWR | 391-0630 | |
50 ml serological pipet | Corning BV | 357550 | |
5 ml serological pipet | Corning BV | 357543 |
Reagent | Volume | Final concentration in reaction | |
Water | 495 ml | ||
20x SSC buffer | 2.5 ml | 0.1x | |
20% SDS solution | 2.5 ml | 0.1% | |
sum | 500 ml |