Mar 04, 2025

Public workspace Striatal Punch Collection

DOI
dx.doi.org/10.17504/protocols.io.dm6gp97xdvzp/v1
  • Katerina Rademacher1,
  • Ken Nakamura1
  • 1Gladstone Institutes
James Maas
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DOI: dx.doi.org/10.17504/protocols.io.dm6gp97xdvzp/v1
Protocol Citation: Katerina Rademacher, Ken Nakamura 2025. Striatal Punch Collection. protocols.io https://dx.doi.org/10.17504/protocols.io.dm6gp97xdvzp/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 27, 2025
Last Modified: March 05, 2025
Protocol Integer ID: 123561
Keywords: ASAPCRN
Funders Acknowledgements:
ASAP
Grant ID: 025184
Abstract
This protocol describes how to collect striatal punches from fresh frozen mouse brain tissue for HPLC analysis.
Striatal Punch Collection
Striatal Punch Collection
Sacrifice mice with CO2, rapidly remove the brain, and freeze in superchilled isopentane on dry ice. Store brains at -80˚C until ready to collect tissue.
Embed brains in OCT, and mount on a cryostat. Trim until the striatum, then take punches from the dorsal striatum using a pipette tip cut to 1mm in diameter.
Store striatal punches at -80˚C until ready to ship for analysis. Ship on dry ice.