The animal pole of the embryo is established at the site of sperm entry, and at 20°C the first cell syncitium is usually visible within 45 minutes. The first cell division usually occurs approximately 25 minutes later, and the two cell stage is fully visible approximately 1.5 hours post fertilization (hpf). During this time, the chorion thickens and attaches to the petri dish as well as to other embryos. At or after 2 hpf, use the wide blade entomological forceps to detach the embryos from one another, as well as to dislodge embryos from the bottom of the petri dish. Remove all unfertilized eggs to limit mold contamination, and record the total number of eggs and embryos. Rinse embryos 3 – 4 X with SBEM, then distribute 30-50 embryos to 90 mm petri dishes with about 100 ml fresh SBEM. Enter cross information into database, print labels for dishes, and place embryos into incubator at 20°C.