Nov 20, 2024

Public workspaceStedehouder, J. and Roberts, B.M. et al. (2024) Rapid modulation of striatal cholinergic interneurons and dopamine release by satellite astrocytes V.2

DOI
dx.doi.org/10.17504/protocols.io.dm6gp3k25vzp/v2
  • 1Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford OX1 3PT, UK;
  • 2Oxford Parkinson’s Disease Centre, University of Oxford, Oxford OX1 3PT, UK;
  • 3Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD 20815, USA;
  • 4Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford OX3 9DU, UK;
  • 5Département de Neurosciences, Université de Montréal, Montréal, Québec, Canada;
  • 6Centre Interdisciplinaire de Recherche sur le Cerveau et l’Apprentissage, Université de Montréal, Montréal, Québec, Canada;
  • 7Faculté de Médecine Dentaire, Université de Montréal, Montréal, Québec, Canada
  • Jeff Stedehouder: currently based at: Medical Research Council Brain Network Dynamics Unit, Nuffield Department of Clinical Neurosciences, University of Oxford, Mansfield Road, Oxford OX1 3TH, United Kingdom;
  • Bradley M. Roberts: currently based at: UK Dementia Research Institute, University of Cambridge, Cambridge UK;
Cláudia C. Mendes
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DOI: dx.doi.org/10.17504/protocols.io.dm6gp3k25vzp/v2
Collection Citation: Jeff Stedehouder, Bradley M. Roberts, Shinil Raina, Alan K. L. Liu, Laura Parkkinen, Dorly Verdier, Arlette Kolta, Stephanie J Cragg 2024. Stedehouder, J. and Roberts, B.M. et al. (2024) Rapid modulation of striatal cholinergic interneurons and dopamine release by satellite astrocytes. protocols.io https://dx.doi.org/10.17504/protocols.io.dm6gp3k25vzp/v2Version created by Cláudia C. Mendes
Manuscript citation:
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: January 26, 2024
Last Modified: November 20, 2024
Collection Integer ID: 112427
Keywords: astrocytes, cholinergic interneurons, acetylcholine
Funders Acknowledgement:
Aligning Science Across Parkinson’s
Grant ID: ASAP-020370
Medical Research Council
Grant ID: MR/V013599/1
Medical Research Council
Grant ID: MC_UU_00003/5
Abstract
Files
Protocol
Icon representing the file Making Ca2+ - selective microelectrodes
Name
Making Ca2+ - selective microelectrodes
Version 1
, University of Oxford
Cláudia C. MendesUniversity of Oxford
Protocol
Icon representing the file Immunofluorescent Labelling of Post-Mortem Rodent Brain Tissue
Name
Immunofluorescent Labelling of Post-Mortem Rodent Brain Tissue
Version 1
, University of Oxford
Cláudia C. MendesUniversity of Oxford
Protocol
Icon representing the file Intracranial injections of viral vectors in mouse striatum
Name
Intracranial injections of viral vectors in mouse striatum
Version 1
, University of Oxford
Cláudia C. MendesUniversity of Oxford
Protocol
Icon representing the file Fast-scan cyclic voltammetry to assess dopamine release in ex vivo mouse brain slices while optogenetically activating astrocytes
Name
ForkFast-scan cyclic voltammetry to assess dopamine release in ex vivo mouse brain slices while optogenetically activating astrocytes
Version 1
, University of Oxford
Shinil RainaUniversity of Oxford
Protocol
Icon representing the file GRAB Acetylcholine (ACh) sensor imaging in ex vivo mouse striatal slices
Name
ForkGRAB Acetylcholine (ACh) sensor imaging in ex vivo mouse striatal slices
Version 1
, University of Oxford
Cláudia C. MendesUniversity of Oxford
Protocol
Icon representing the file Imaging of cholinergic interneurons in post-mortem rodent tissue to identify striatal satellite astrocytes
Name
Imaging of cholinergic interneurons in post-mortem rodent tissue to identify striatal satellite astrocytes
Version 1
, University of Oxford
Cláudia C. MendesUniversity of Oxford
Protocol
Icon representing the file Immunofluorescence labelling and imaging of cholinergic interneurons in post-mortem human brain tissues
Name
Immunofluorescence labelling and imaging of cholinergic interneurons in post-mortem human brain tissues
Version 1
, University of Oxford
Cláudia C. MendesUniversity of Oxford
Protocol
Icon representing the file Whole-cell Patch-Clamp Recordings from Striatal Cholinergic Interneurons in ex vivo Mouse Brain Slices
Name
Whole-cell Patch-Clamp Recordings from Striatal Cholinergic Interneurons in ex vivo Mouse Brain Slices
Version 2
, University of Oxford
Cláudia C. MendesUniversity of Oxford
Protocol references