Dec 12, 2024

Public workspaceSTC-1 cell culture

 Forked from STC-1 cell culture
DOI
dx.doi.org/10.17504/protocols.io.j8nlk98kwv5r/v1
  • 1Department of Clinical and Movement Neurosciences, UCL Queen Square Institute of Neurology, London, UK;
  • 2Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, Maryland, USA.
Alexandria White
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DOI: dx.doi.org/10.17504/protocols.io.j8nlk98kwv5r/v1
Protocol Citation: Michael J Hurley 2024. STC-1 cell culture. protocols.io https://dx.doi.org/10.17504/protocols.io.j8nlk98kwv5r/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 11, 2024
Last Modified: December 12, 2024
Protocol Integer ID: 114725
Keywords: ASAPCRN
Funders Acknowledgements:
Aligning Science Across Parkinson’s
Grant ID: ASAP-000420
Abstract
Cell culture of STC-1 mouse enteroendocrine cells.
STC-1 cell culture
STC-1 cell culture
Obtain Mouse (Mus musculus) neuroendocrine duodenal adenoma STC-1 cells (RRID:CVCL_J405) from the American Type Culture Collection (ATCC-CRL-3254™).
Grow STC-1 cells in DMEM:F12 Ham with GlutaMax™ media supplemented with 10 % heat-inactivated fetal bovine serum (Gibco) and penicillin (100 U/ml) streptomycin (100 mg/ml) on 24 or 96 well plates at 37°C under saturating humidity in a 5% CO2/95% air mixture.
Subculture cells when 80-90 % confluent using Trypsin and replate at 1:10 dilution for maintenance or at an appropriate density for experiments.