Apr 02, 2023

Public workspaceSTC-1 cell culture

DOI
dx.doi.org/10.17504/protocols.io.5jyl8jky8g2w/v1
  • 1Department of Clinical and Movement Neurosciences, UCL Queen Square Institute of Neurology, London, UK;
  • 2Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, Maryland, USA.
Michael J Hurley
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DOI: dx.doi.org/10.17504/protocols.io.5jyl8jky8g2w/v1
Protocol Citation: Michael J Hurley 2023. STC-1 cell culture. protocols.io https://dx.doi.org/10.17504/protocols.io.5jyl8jky8g2w/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 27, 2023
Last Modified: May 31, 2024
Protocol Integer ID: 77704
Keywords: ASAPCRN
Funders Acknowledgement:
Aligning Science Across Parkinson’s
Grant ID: ASAP-000420
Abstract
Cell culture of STC-1 mouse enteroendocrine cells.
STC-1 cell culture
STC-1 cell culture
Obtain Mouse (Mus musculus) neuroendocrine duodenal adenoma STC-1 cells (RRID:CVCL_J405) from the American Type Culture Collection (ATCC-CRL-3254™).
Grow STC-1 cells in DMEM:F12 Ham with GlutaMax™ media supplemented with 10 % charcoal absorbed foetal bovine serum (LabTech), 1 mM sodium pyruvate, non-essential amino acids (100 mM) and penicillin (100 U/ml) streptomycin (100 mg/ml) on 12 well plates at 37°C under saturating humidity in a 5% CO2/95% air mixture.
Subculture cells when 80-90 % confluent using Accutase™ and replate at 1:10 dilution for maintenance or at an appropriate density for experiments.