• Anesthetics (e.g. isoflurane) and induction chamber
o 2 charcoal filter canisters for isoflurane output
o Necessary tubing to connect equipment
• Stereotaxic surgery frame
• Drill with 0.5 mm drill bit, power supply, and foot pedal
• Warming pad, anal probe, and power supply
• Nanoliter injector (NANOLOTER-2020, WPI)
• Non-steroidal analgesic (e.g. carprofen)
• Antiseptic solution for skin (e.g., povidone-iodine solution and 70% ethanol)
• Sterile ophthalmic ointment
• Sterile surgical instruments
• Sterile swabs and gelfoam spears
• Glass pipettes for injection