Flowmi cell strainers, 40 µm (Bel-Art, cat no. H13680-0040)
DNA LoBind microcentrifuge tubes, 1.5 mL (Eppendorf, cat no. 0030 108 418)
Round-bottom polypropylene tubes with cap, 5 mL (Falcon, cat no. 352063)
Wide bore pipette tips, 1000 μL (Axygen, cat no. T-1005-WB-C) (optional)
Micropipettes DV1000, DV200, DV20, D10 (THL, cat no. PZ-7901)
Cell strainers, 70 µm (Corning, cat no. 431751)
Tweezers with pointed tips, 115mm (Weller Erem, cat no. 5SA)
Tissue-culture treated culture dishes, 35 x 10 mm (Corning, cat no. 430165)
Thin wall PCR tubes with flat cap, 200 µL (Axygen, cat no. PCR-02-L-C)
96-well plate with round bottom (Greiner)
Sterile syringe 0.2 µm filters, acrodisc (Pall, cat no. 2415600)
50 mL syringe
50 mL centrifuge tube
2 mL plastic syringe (BD, cat. no. 302204)
One wild-type male mouse six weeks or older. This protocol was developed using C57BL/6J, FVB/N, and F1(C57BL/6J x FVB/N) mice.